EXPERIMENTS

The objectives of the ExplorEcolab experiment are:

1) recreate the symbiosis butterfly-plants-ants in the laboratory;

2) apply different climate change scenarios in the laboratory to better understand the climate-symbiosis relationship.

To achieve these two objectives, different experiments are explained below.

Experiment 1: Integration of host plants of the Polyommatus icarus butterfly into the laboratory

  • Identification of a Trifolium repens, Lotus corniculatus meadow at Grand-Chemin (44, France) for the collection of plant specimens
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Prairie à Trifolium repens, Lotus corniculatus, Medicago lupulina
  • Species identification
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Flowers of Trifolium repens (c) F. Mallard
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Flowers of Lotus corniculatus (c) F. Mallard
  • Collection of clumps of soil with targeted species
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  • Sampling of specimens in clumps and planting of plants in the laboratory
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  • Seed seeds in July 2019
  • After one month in August 2019
  • After 2 monts in September 2019
  • Development of white spots on plant leaves and small black dots corresponding to the development of small plant parasitic insects called thrips
  • Thrips control test with blue sticky traps
  • Thrips control failure: replacement of substrate with a new sterilized substrate in January 2020
  • In March 2020 : Seed of seeds harvested the year before in 2019